VIB Technologies Nucleomics Core

Library/Pool Submission (Short reads only)

Sample requirements

Quality

Only when samples meet our internal quality criteria, we can guarantee good quality data. These criteria are:

  • Nanodrop OD 260/280 > 1.8

  • Nanodrop OD 260/230 > 1.8
  • Please pay attention that the peak of the Nanodrop profile is situated at 260 nm and not at 270 nm (this would indicate a phenol-like contamination)
  • Note that over 30 samples, you can provide your samples in a PCR plate in column orientation instead of eppendorf tubes.

Please always provide 3-5 µL extra which is to be used in our quality control steps.

Amount

Note that we need higher loading concentrations for the AVITI device compared to Illumina sequencing platforms. Please provide around 30µl of a ~20nM pool.

Please contact us at nucleomics.lab@vib.be for more details.

 

Sample submission

Before sending/bringing your samples, please fill out the library/pool sample submission form and email it to nucleomics.lab@vib.be.

 

Library/Pool Sample Submission form

Submission Process

Step 1

Check Sample Requirements

For sequencing material using long reads, please contact us to discuss specific requirements.

 
Step 2

Fill out the sample submission form

Please, always download the latest version instead of using a previously saved version. ​​​​​​​

Step 3

Email it to nucleomics.lab@vib.be

Step 4

Ship your samples to the VIB Nucleomics Core

/en/shipping-samples