Transcriptomic assays

Historically the vast majority of genome wide expression analysis data have been obtained through microarray experiments. VIB Nucleomics Core has a long standing experience of more than 15 years in this field using initially microarrays (Affymetrix and Agilent), but also the nCounter® technology.

In the past few years, novel sequencing technologies have emerged that enable the analysis of millions of molecules in parallel. With this increase in sequencing throughput and associated drop in per base sequencing cost, expression analysis by Massive Parallel Sequencing (MPS) is now coming into reach. With currently available MPS technologies, RNA needs to be converted into DNA, but direct RNA sequencing technologies are well under way; needless to mention that the VIB Nucleomics Core is closely monitoring these developments. Apart from quantitative information on expression levels, RNA sequencing also enables mutation and structural variation analysis (e.g., novel fusion transcripts and alternative splicing), not only of the coding RNA, but also of the different classes of non-coding RNA, such as miRNA (microRNA) and lincRNA (long interfering non-coding RNA).

Platforms offered:

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