Sample submission

Sample requirements

Only when samples meet our internal quality criteria, we can guarantee good quality data. These criteria are:

For RNA

  • Nanodrop OD 260/280 > 1.8
  • Nanodrop OD 260/230 > 1.8
  • Please pay attention that the peak of the Nanodrop profile is situated at 260 nm and not at 270 nm (this would indicate a phenol-like contamination)
  • Agilent Bioanalyzer RIN (RNA Integrity Number) > 7-8

For DNA

  • Nanodrop OD 260/280 > 1.8
  • Nanodrop OD 260/230 > 1.8
  • Please pay attention that the peak of the Nanodrop profile is situated at 260 nm and not at 270 nm (this would indicate a phenol-like contamination)

Amount

An amount of 100 ng of total RNA is enough for the majority of the RNA applications. For DNA applications, 500 ng of genomic DNA will usually be sufficient. Please contact us at nucleomics@vib.be for more details.

Please always provide 3-5 µL extra which is to be used in our quality control steps.

Sample submission

Before sending/bringing your samples please fill out the appropriate sample submission form and email it to nucleomics.lab@vib.be.

Sequencing experiments on Illumina MiSeq/NextSeq/HiSeq platform:

RNA sample submission form: xls-format or xlsx format
DNA sample submission form: xls-format or xlsx format
Library/Pool submission form: xls-format or xlsx format

BioNano Genomics optical mapping experiments:

DNA-Plug submission form: xls-format or xlsx format
Plant samples submission form: xls-format or xlsx format
shipping instructions submission form: PDF file

Sequencing experiments on the PacificBiosciences Sequel platform:

Please use the Illumina sample submission forms above for the time being. Edits will soon be made to reflect Sequel-specific requirements. Do not hesitate to contact us to discuss requirement details.

Please, always download the latest version instead of using a previously saved version. 

To optimize the workflow, please pay attention to the following:

  • use 1.5 mL eppendorf tubes
  • place your samples in the sample box in the same order as listed in the sample submission form
  • mark each sample tube with a unique name, number, and date matching the details that you fill out on the sample submission form
  • if you have used a specific elution buffer, please include an aliquot of the buffer to be used as blanc for our QC measurements
  • Ship your samples on plenty of dry-ice and preferably on the beginning of the week. Pay attention to our lab closing days, listed on the right side of this web-page. Please use the following address to ship your samples to:

VIB Nucleomics Core
Herestraat 49, O&N4, Post Box 816
Room nr. 404-24 / 08.428
3000 Leuven – Belgium

Popups Powered By : XYZScripts.com