Massive Parallel Sequencing

 

Illumina instruments @VIB NC

VIB Nucleomics Core offers Illumina Next Generation Sequencing platforms, with great flexibility in running both small and large scale projects within a reasonable time.

Available Instruments at the Nucleomics Core

  • Illumina MiSeq instrument (Info  Specs)
  • Illumina NextSeq 500 instrument (Info  Specs)
  • Illumina HiSeq 4000 instrument (Info  Specs)
  • Illumina NovaSeq 6000 instrument (Info  Specs)

Among the most appropriate applications

  • Whole-Genome sequencing
  • Exome Sequencing
  • Target Sequencing (amplicon)
  • 16S Metagenomics Sequencing
  • ChIP-seq
  • 10X Genomics single cell libraries
  • Whole-Transcriptome Sequencing (mRNA and total RNA-seq)
  • Gene Expression Profiling
  • Small RNA sequencing (small RNA-seq)

See more on Illumina webpages

Sequencing library preparation

Steps in each sequencing project, irrespective of the platform or application:

  1. library preparation
  2. bridge amplification
  3. the sequencing

The sequencing library preparation is highly dependent on the application and often multiple vendors are offering slightly different library preparation kits. VIB Nucleomics Core is constantly implementing these library preparation protocols, preferentially on an automated platform. Especially with targeted resequencing, many options are available to enrich for sequences of interest to the researcher. PCR based approaches are very well suited for the smaller target regions (< 200 kbp).

Companies offering PCR based enrichment solutions:

Outsourcing

Apart from running sequencing services in-house on Illumina or PacBio, VIB Nucleomics Core, through its contacts with other service providers or its Core4Life network, also can offer project management involving outsourcing to established companies like Complete Genomics, BGI or for evaluation to technology providers building novel platforms (e.g.  Oxford nanopore technology).

Experimental design, sample requirements, and data analysis expectations

Clearly, sequencing is currently a rapidly evolving field with already now a wealth of different applications and options to the researcher.   Therefore we highly recommend to contact us and to consult us on experimental design, sample requirements, and data analysis expectations.

Sample submission

 

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